The evidence, including the parts that did not work.
We register protocols before enrolment and publish the endpoints that failed alongside the ones that did not. This page is the whole of it.
Four registered studies · 1,552 participants · Protocols published in full
How the capsule survives the stomach.
A capsule inside a capsule. The outer shell carries the prebiotic and dissolves early; the inner core stays shut until it is past the acid, which is where the eleven-fold survival figure comes from.
- Outer shell
- Dissolves at pH 5.5
- Prebiotic layer
- 400mg polyphenol
- Inner core
- 24 strains, 53.6B AFU
- Delayed seam
- Opens past the stomach
Cross-section, TN-01 Daily Synbiotic. Drawn to scale from the manufacturing specification.
Four studies, and what each one was for.
Protocols were registered before enrolment. Where an endpoint did not reach significance, it is reported in the paper alongside the ones that did.
-
Twelve-week synbiotic trial
228 adults · 2023
Randomised, double-blind, placebo-controlled
The primary endpoint was self-reported bloating at week twelve. Secondary endpoints covered transit time, stool consistency and skin hydration. The full protocol was registered before the first participant enrolled.
-
Capsule survival model
In vitro · 2023
Simulated gastric and intestinal digestion
The capsule-in-capsule was compared against an uncoated control through a two-stage digestion model. Eleven times more organisms survived to the intestinal stage.
-
Glycine and next-day alertness
84 adults · 2024
Crossover, placebo-controlled
Sleep onset latency and next-morning alertness were measured together, because a sleep aid that improves one at the cost of the other has not worked.
-
Micronutrient gap survey
1,240 diet records · 2022
Observational, pre-formulation
We used it to decide what to leave out. Twelve nutrients showed a meaningful shortfall; the rest went in the bin rather than on the label.
Findings, in the order they were measured.
Each row names its study and its sample size. The full protocol for every one of them is downloadable, without an email address.
- 89 %
- reported less bloating after twelve weeks
- TN-01, n=228, randomised, placebo-controlled
- 11 ×
- more strains survived to the colon than the uncoated control
- In vitro digestion model, 2023
- 3.2 d
- faster return to regular transit time
- TN-01, n=228, versus placebo arm
- 24
- strains, each held at the dose its own trial used
- Formulation record, published in full
- 0
- proprietary blends anywhere in the range
- Every amount is printed on the jar
Not sure where to start?
Four questions, one recommendation, no email required to see it.
Take the two-minute quiz
Read before you buy.
The protocol, the endpoints that failed, and the sourcing limits — all of it, in one place.
Open the evidence